Burnout is a state of physical, mental and emotional exhaustion. Research shows that burnout affects not only physical and mental health, but behavior as well. Aim of the present study is to assess burnout and its relationship with aggression in amateur athletes’ performances in university games. Methods: In this empirical study 169 amateur athletes were selected from Sindh University, Jamshoro using the Convenient Sampling Technique. Two structured questionnaires Buss-Perry Aggression Questionnaire (BAQ) and Athlete Burnout Questionnaire (ABQ) were used to collect data along with demographic sheet. The results of simple linear regression indicated that aggression predicts burnout (R2 = .043, F = 7.49, p = .007.). However, no significant gender differences were observed in aggression and burnout. Conclusion: Burnout is strongly correlated with mental health including irregular and unmonitored onset of aggressive behavior of amateur athletes. The negative effects of burnout at the individual, collective and organizational level prompt researchers to look for the specific factors associated with the syndrome that seem to influence its occurrence.