The basic purpose of current work is to determine the effect of the Mastery Learning Approaches (MLA) on improving students’ academic performance in science, as well as their effect on improving students’ learning outcomes. These were conducted in order to determine the best methods of teaching for the improvement of students’ science learning outcomes. The non-equivalent post-test, pre-test experimental design was used in this study. In Multan tehsil Saddar, 43 basic educational elementary schools were chosen using a simple random sampling technique. According to the study, MLA can help improving students’ learning outcomes in science. It is thus recommended that the Education Ministry should conduct trainings for science teachers on how to effectively implement these pioneering teaching tactics during instruction so that students can be guided to learn meaningfully and are helped in remembering, what they have learned in science.